QHow exactly is the beet H7-1 beet genetically modified? Is a plasmid added to the DNA?

How exactly is the beet H7-1 beet genetically modified? Is a plasmid added to the DNA?

AExpert Answer

Roundup Ready sugar beets H7-1 was developed by first making a piece of DNA, called an expression cassette, that contains the cp4 epsps gene for tolerance to the herbicide glyphosate, which is the active ingredient in Roundup agricultural herbicides. Using genetic-engineering techniques, that expression cassette is spliced into a plasmid and transformed into Agrobacterium. The Agrobacterium is then added to a petri dish containing sugar beet cells growing in tissue culture. The Agrobacterium uses a natural process to insert that expression cassette into the genome of the plant. The DNA of the resulting plant then contains the cp4 epsps gene and has a tolerance to glyphosate. The process is not unique to sugar beets and is used to develop most GM plants. A detailed description of the process is available in the USDA petition, starting on page 19: http://www.aphis.usda.gov/brs/aphisdocs/03_32301p.pdf

 

There are also several YouTube videos that show the basic process for making GM plants using the Agrobacterium method.

 

 

 

Posted on January 31, 2018
Thank you for your question. There are various aspects of your question. I assume your question refers to the use of Agrobacterium rhizogenes by scientists to intentionally transfer genes from the bacterium to plants. Infection and DNA transfer from this bacterium occurs in nature all the time to cause disease. Such transformed plants are not classified as GMOs since transfer occurred naturally. If this is done by scientists then it would be classified as a GMO. Rules and... Read More
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Posted on March 1, 2018
I’m a Monsanto scientist who has more than 20 years of experience with genetic modification of plants. I will try to answer your question, even though I don’t ever do experiments on animals, certainly not on humans, of course! Can humans be genetically modified…but a much bigger question is should humans be genetically modified? There are two ways to think about genetic modification of humans (or any animal). One way is modification of somatic cells, and the other is the... Read More
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Posted on May 10, 2017
The simple answer is that 20+ years of composition assessments of GMO crops have demonstrated that crop composition is not appreciably affected by the GM process (1). In addition, data collected through that time have indicated that general factors such as the growth environment can contribute to notable variation in component levels (2). Plant agglutinins (or lectins) and amylase inhibitors are examples of anti-nutritional compounds that may be present in crops. The relevance of such a... Read More