QHow exactly is the beet H7-1 beet genetically modified? Is a plasmid added to the DNA?

How exactly is the beet H7-1 beet genetically modified? Is a plasmid added to the DNA?

AExpert Answer

Roundup Ready sugar beets H7-1 was developed by first making a piece of DNA, called an expression cassette, that contains the cp4 epsps gene for tolerance to the herbicide glyphosate, which is the active ingredient in Roundup agricultural herbicides. Using genetic-engineering techniques, that expression cassette is spliced into a plasmid and transformed into Agrobacterium. The Agrobacterium is then added to a petri dish containing sugar beet cells growing in tissue culture. The Agrobacterium uses a natural process to insert that expression cassette into the genome of the plant. The DNA of the resulting plant then contains the cp4 epsps gene and has a tolerance to glyphosate. The process is not unique to sugar beets and is used to develop most GM plants. A detailed description of the process is available in the USDA petition, starting on page 19: http://www.aphis.usda.gov/brs/aphisdocs/03_32301p.pdf


There are also several YouTube videos that show the basic process for making GM plants using the Agrobacterium method.




Posted on July 21, 2017
GMOs aren't really added directly to the meat, beef.  However, beef cattle may consume feed that comes from a genetically modified plant. All beef cattle begin their lives on a farm or ranch, grazing pasture or grass - none of which is considered a GMO. For many cows this will be their sole source of feed for their lifetime. Some cattle receive rations of grain, which may contain corn or soybeans, both of which have genetically modified hybrids and varieties. ... Read More
Posted on March 28, 2017
Thanks for the question, which I will address in two ways here.   1. What are three ways that organisms are modified by scientists? Here I will focus only on plants.   a. Agrobacterium: Agrobacterium tumefaciens (Agro) is a naturally occurring soil organism that causes a disease in plants called crown gall disease. In the late 1970s, Mary-Dell Chilton discovered that Agro actually transfers genes (DNA) from the Agro to the plant cell, where it becomes integrated into the plant... Read More
Posted on March 2, 2017
First of all, to clarify – hybridization is part of conventional breeding and conventional breeding uses hybridization to create new combinations of genes from parent varieties. For example, a disease-resistant wheat variety may be hybridized to a variety that makes flour better suited for making whole wheat bread. This is a common goal of most conventional breeding programs. It typically involves taking pollen from one parent and using it to fertilize another parent. The... Read More