QMy question pertains to the genetic modification of Roundup Ready soybeans. How is it that this process occurs? Id like to know the specific steps used from the targeting of specific genes at the start to the finished product. Thank you

My question pertains to the genetic modification of Roundup Ready soybeans. How is it that this process occurs? Id like to know the specific steps used from the targeting of specific genes at the start to the finished product. Thank you

AExpert Answer

The basic steps are as follows:

 

  1. Identify a gene that will provide resistance to the herbicide.
  2. Based on the sequence of the resistant gene, make a DNA expression cassette that contains a promoter to turn on the gene, the resistant gene, and a sequence to stop gene transcription. 
  3. Transform the plant in tissue culture to incorporate the resistant gene
  4. Screen for plants that have successfully incorporated the resistant gene and are tolerant to glyphosate.

A very good detailed description of the process is already available at http://sandwalk.blogspot.com/2007/03/roundup-ready-transgenic-plants.html.  Additional details about Roundup Ready soybeans are also available in the USDA petition for deregulation at http://www.aphis.usda.gov/brs/aphisdocs/93_25801p.pdf.

 

If you have additional questions, I would be happy to answer them. 

Posted on March 2, 2017
The term “GMO” typically refers to crops or animals that, through genetic engineering, have had a gene (or a few genes) from a different species inserted into their genome. So yes, by design, to improve a crop or animal with genetic engineering, the genome of the new, GE variety has been changed by the addition of new genes(s).     Your question also asks about whether inserting the new gene(s) will “…activate genes…” Some traits in... Read More
Posted on August 15, 2017
The first use of recombinant DNA technology, was created by Cohen and Boyer in 1972 with E.coli in 1972 and this article explains this advancement in biotechnology in greater detail. Here is an excerpt: “Their experiments dramatically demonstrated the potential impact of DNA recombinant engineering on medicine and pharmacology, industry and agriculture.”   Recombinant insulin was the first commercial product derived from genetic engineering techniques created in 1976 by the... Read More
Posted on May 6, 2017
A gene with a desirable trait can be moved from one organism to another organism as a means to change it. The traditional way is through selective breeding, which is slow, time consuming, inefficient, and transfers more than one gene, so other unexpected and unwanted traits can cause problems. But genes also can be moved in a laboratory, resulting in what has been called a genetically modified (“transgenic”) organism (GMO). GM technology moves only one gene, eliminating other,... Read More
Answer: