QMy question pertains to the genetic modification of Roundup Ready soybeans. How is it that this process occurs? Id like to know the specific steps used from the targeting of specific genes at the start to the finished product. Thank you

My question pertains to the genetic modification of Roundup Ready soybeans. How is it that this process occurs? Id like to know the specific steps used from the targeting of specific genes at the start to the finished product. Thank you

AExpert Answer

The basic steps are as follows:

 

  1. Identify a gene that will provide resistance to the herbicide.
  2. Based on the sequence of the resistant gene, make a DNA expression cassette that contains a promoter to turn on the gene, the resistant gene, and a sequence to stop gene transcription. 
  3. Transform the plant in tissue culture to incorporate the resistant gene
  4. Screen for plants that have successfully incorporated the resistant gene and are tolerant to glyphosate.

A very good detailed description of the process is already available at http://sandwalk.blogspot.com/2007/03/roundup-ready-transgenic-plants.html.  Additional details about Roundup Ready soybeans are also available in the USDA petition for deregulation at http://www.aphis.usda.gov/brs/aphisdocs/93_25801p.pdf.

 

If you have additional questions, I would be happy to answer them. 

Posted on January 31, 2018
Thank you for your question. There are various aspects of your question. I assume your question refers to the use of Agrobacterium rhizogenes by scientists to intentionally transfer genes from the bacterium to plants. Infection and DNA transfer from this bacterium occurs in nature all the time to cause disease. Such transformed plants are not classified as GMOs since transfer occurred naturally. If this is done by scientists then it would be classified as a GMO. Rules and... Read More
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Posted on March 1, 2018
I’m a Monsanto scientist who has more than 20 years of experience with genetic modification of plants. I will try to answer your question, even though I don’t ever do experiments on animals, certainly not on humans, of course! Can humans be genetically modified…but a much bigger question is should humans be genetically modified? There are two ways to think about genetic modification of humans (or any animal). One way is modification of somatic cells, and the other is the... Read More
Answer:
Posted on May 10, 2017
The simple answer is that 20+ years of composition assessments of GMO crops have demonstrated that crop composition is not appreciably affected by the GM process (1). In addition, data collected through that time have indicated that general factors such as the growth environment can contribute to notable variation in component levels (2). Plant agglutinins (or lectins) and amylase inhibitors are examples of anti-nutritional compounds that may be present in crops. The relevance of such a... Read More